This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLEX307-mFzd4
catalog :
102866
citations: 1
Reference
Susman M, Karuna E, Kunz R, Gujral T, Cantú A, Choi S, et al. Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates. elife. 2017;6: pubmed publisher
product information
Catalog Number :
102866
Product Name :
pLEX307-mFzd4
article :
doi10.7554/eLife.26509
id28191854
pubmed_id28885975
bacterial resistance :
Ampicillin
cloning :
backbonepLEX_307
backbone_mutation
backbone_originAddgene #41392 (provided by David Root)
backbone_size10065
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses mouse Fzd4 in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterEF1A
sequencing_primer_3
sequencing_primer_5EF1A promoter forward (TCAAGCCTCAGACAGTGGTTC)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFz4
geneFzd4
id14366
genbank_ids
mutationT to C substitution at nucleotide 1149 of Fzd4 ORF. C to T substitution at nucleotide 1248 of Fzd4 ORF. Both substitutions are silent.
nameFrizzled 4
shRNA_sequence
size1614
species
10090
Mus musculus
tags
plasmid copy :
Insert was PCR amplified from Addgene plasmid #42256 (provided by Jeremy Nathans and Chris Garcia) and subcloned into a modified pENTR-2B vector using FseI and AscI sites.
resistance markers :
3641
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA