This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDH-puro-EWS-FLI1
catalog :
102813
citations: 5
Reference
Tulpule A, Menon S, Breese M, Lin Y, Allegakoen H, Perati S, et al. FET fusion oncoproteins disrupt physiologic DNA repair networks and induce ATR synthetic lethality in cancer. Res Sq. 2023;: pubmed publisher
Menon S, Breese M, Lin Y, Allegakoen H, Perati S, Heslin A, et al. FET fusion oncoproteins disrupt physiologic DNA repair networks in cancer. bioRxiv. 2023;: pubmed publisher
Gao Y, He X, Wu X, Huang Y, Toneyan S, Ha T, et al. ETV6 dependency in Ewing sarcoma by antagonism of EWS-FLI1-mediated enhancer activation. Nat Cell Biol. 2023;25:298-308 pubmed publisher
Liang Q, Zheng Q, Zuo Y, Chen Y, Ma J, Ni P, et al. SENP2 Suppresses Necdin Expression to Promote Brown Adipocyte Differentiation. Cell Rep. 2019;28:2004-2011.e4 pubmed publisher
Loganathan S, Tang N, Fleming J, Ma Y, Guo Y, Borinstein S, et al. BET bromodomain inhibitors suppress EWS-FLI1-dependent transcription and the IGF1 autocrine mechanism in Ewing sarcoma. Oncotarget. 2016;7:43504-43517 pubmed publisher
product information
Catalog Number :
102813
Product Name :
pCDH-puro-EWS-FLI1
article :
doi10.18632/oncotarget.9762
id28192021
pubmed_id27259270
bacterial resistance :
Ampicillin
cloning :
backbonepCDH-puro
backbone_mutation
backbone_origin
backbone_size7384
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The cDNA sequence of EWS-FLI1 was subcloned into the NheI and NotI sites of the lentiviral vector pCDH-CMV-MCS-EF1-Puro.
growth strain :
Overexpress EWS-FLI1. 3rd generation lentiviral vector.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Not 1
cloning_site_5Nhe 1
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEWS, EWS-FLI1, bK984G1.4
geneEWSR1
id2130
genbank_ids
mutation
nameEWS-FLI1
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
1651
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA