This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Rab5-L38R-mCherry
catalog :
102785
product information
Catalog Number :
102785
Product Name :
Rab5-L38R-mCherry
article :
| doi | |
| id | 28192019 |
| pubmed_id |
bacterial resistance :
Kanamycin
cloning :
| backbone | EGFP-C1 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | 4731 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
mCherry-C2-Rab5a encodes wild-type human Rab5a,(NM_004162) as a fusion protein with mCherry. Rab5a was inserted between the Eco R1 and Sal 1 restriction sites. mCherry-C2 encodes a monomeric variant of DsRed. The backbone plasmid is pEGFP-C1 (Clontech). The C2 version of mCherry was created by insertion of a short piece of DNA between Xho 1 and EcoR1 restriction sites. In the resulting multiple cloning site, the Sac 1 restriction site has been eliminated and the Eco R1 restriction site is in frame.
growth strain :
Point mutation in Rab5 that reestablishes interaction with APPL1 when co-expressed with APPL1-N308D
origin :
37
pi :
|
resistance markers :
| 2063 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments