This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIG-Flag-RIPK1-wt
catalog :
102677
citations: 1
Reference
Zhang L, Blackwell K, Workman L, Chen S, Pope M, Janz S, et al. RIP1 Cleavage in the Kinase Domain Regulates TRAIL-Induced NF-κB Activation and Lymphoma Survival. Mol Cell Biol. 2015;35:3324-38 pubmed publisher
product information
Catalog Number :
102677
Product Name :
pMIG-Flag-RIPK1-wt
article :
doi10.1128/MCB.00692-15
id28191979
pubmed_id26195820
bacterial resistance :
Ampicillin
cloning :
backbonepMIG
backbone_mutation
backbone_origin
backbone_size7166
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The RIPK1 insert has a K87Q polymorphism which has no effect on protein activity.
growth strain :
retroviral expression plasmid for RIPK1-wt
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3MfeI/EcoRI
cloning_site_5Bgl II
promoterunknown
sequencing_primer_3
sequencing_primer_5pLXSN 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAIEFL, IMD57, RIP, RIP-1, RIP1
geneRIPK1
id8737
genbank_ids
NM_003804
mutation
nameRIPK1
shRNA_sequence
size2045
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
locationC terminal on backbone
tagIRES-GFP
resistance markers :
3631
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA