This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28a-6XHis-SUMO-SIRT2
catalog :
102622
citations: 1
Reference
Jing H, Hu J, He B, Negrón Abril Y, Stupinski J, Weiser K, et al. A SIRT2-Selective Inhibitor Promotes c-Myc Oncoprotein Degradation and Exhibits Broad Anticancer Activity. Cancer Cell. 2016;29:297-310 pubmed publisher
product information
Catalog Number :
102622
Product Name :
pET28a-6XHis-SUMO-SIRT2
article :
doi10.1016/j.ccell.2016.02.007
id28191961
pubmed_id26977881
bacterial resistance :
Kanamycin
cloning :
backbonepET28a-6XHis-SUMO
backbone_mutation
backbone_origin
backbone_size5633
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification
origin :
37
pi :
alt_names
Sirtuin 2
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoter
sequencing_primer_3T7 terminator, GCTAGTTATTGCTCAGCGG
sequencing_primer_5T7 promoter, TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSIR2, SIR2L, SIR2L2
geneSIRT2
id22933
genbank_ids
NC_000019.10
mutationDeleted amino acids 1-37 and 357-389
nameSIRT2
shRNA_sequence
size984
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6XHis-SUMO
resistance markers :
3464
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA