pCH1/RAD51o | Addgene 102562 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCH1/RAD51o

catalog :

102562

citations: 2

Reference
Subramanyam S, Spies M. Expression, Purification, and Biochemical Evaluation of Human RAD51 Protein. Methods Enzymol. 2018;600:157-178 pubmed publisher
Subramanyam S, Ismail M, Bhattacharya I, Spies M. Tyrosine phosphorylation stimulates activity of human RAD51 recombinase through altered nucleoprotein filament dynamics. Proc Natl Acad Sci U S A. 2016;113:E6045-E6054 pubmed

product information

Catalog Number :

102562

Product Name :

pCH1/RAD51o

article :

doi	10.1073/pnas.1604807113
id	28191946
pubmed_id	27671650

bacterial resistance :

Kanamycin

cloning :

backbone

pCOLADuet-1

backbone_mutation

backbone_origin

Novagen.EMD Millipore

backbone_size

3719

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

This plasmid was specifically designed to allow high levels of RAD51 expression in E.coli along with the ability to incorporate non-natural amino acids using amber suppressor technology. It was used to incorporate an artificial amino acid to mimic tyrosine phosphorylation (published in Subramanyam S, et. al. (2016). Proceedings of the National Academy of Sciences 113(41):E6045-E6054).

growth strain :

Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

growth temp :

we use the plasmid to express RAD51 in E.coli Acella cells (Edge Bio) which have a RecA deletion that is required for purification.

origin :

pi :

alt_names

RAD51

DNA repair protein RAD51 homolog 1

RAD51A

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	NcoI
promoter	T7
sequencing_primer_3	TCGCACCGGCAAAACGCAG
sequencing_primer_5	TCAGAGCCGCTCAGACCATAGC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA
gene	RAD51
id	5888

genbank_ids

NM_002875.4

mutation

Codon optimized for E.coli Expression

name

Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1

shRNA_sequence

size

1020

species

9606

Homo sapiens

100

Synthetic