pSS1 (hLPL-FLAG) | Addgene 102447 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSS1 (hLPL-FLAG)

catalog :

102447

citations: 1

Reference
Chi X, Shetty S, Shows H, Hjelmaas A, Malcolm E, Davies B. Angiopoietin-like 4 Modifies the Interactions between Lipoprotein Lipase and Its Endothelial Cell Transporter GPIHBP1. J Biol Chem. 2015;290:11865-77 pubmed publisher

product information

Catalog Number :

102447

Product Name :

pSS1 (hLPL-FLAG)

article :

doi	10.1074/jbc.M114.623769
id	28191907
pubmed_id	25809481

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA6

backbone_mutation

backbone_origin

backbone_size

5000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expresses FLAG-tagged human LPL in mammalian cells

origin :

pi :

alt_names

LPL

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	BgH polyA reverse
sequencing_primer_5	T7 promoter forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HDLCQ11, LIPD
gene	LPL
id	4023

genbank_ids

mutation

name

Lipoprotein lipase

shRNA_sequence

size

1560

species

9606

Homo sapiens

tags

location	C terminal on insert
tag	FLAG

location	C terminal on insert
tag	6x His

plasmid copy :

LPL insert originally cloned by Mark Doolittle (Ben-Zeev O, Mao HZ, Doolittle MH. Maturation of Lipoprotein Lipase in the Endoplasmic Reticulum CONCURRENT FORMATION OF FUNCTIONAL DIMERS AND INACTIVE AGGREGATES. J Biol Chem. 2002 Mar 22;277(12):10727 10738. PMID: 11796709)

resistance markers :

3876

tags :

High Copy

terms :

Blasticidin