This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mAzami-Green-Galectin
catalog :
102419
citations: 1
Reference
Dingjan I, Verboogen D, Paardekooper L, Revelo N, Sittig S, Visser L, et al. Lipid peroxidation causes endosomal antigen release for cross-presentation. Sci Rep. 2016;6:22064 pubmed publisher
product information
Catalog Number :
102419
Product Name :
mAzami-Green-Galectin
article :
doi10.1038/srep22064
id21038
pubmed_id26907999
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA6-myc his version B
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The his-tag and myc-tag are NOT in frame with the Gal3-mAzami green.
growth strain :
Expresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.
origin :
37
pi :
alt_names
Galectin-3
35 kDa lectin
cloning
clone_methodRestriction Enzyme
cloning_site_3PspOMI
cloning_site_5NotI
promoterCMV
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2
geneLGALS3
id3958
genbank_ids
NM_002306.3
mutation
nameLGALS3
shRNA_sequence
size765
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmAzami-green
plasmid copy :
The insert is from plasmid mAG-GAL3 (Addgene plasmid #62734), from the lab of Niels Geijsen and has been described in [D Astolfo, D. S. et al. Efficient intracellular delivery of native proteins. Cell 161, 674 690 (2015)].
resistance markers :
3431
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA