pSIN-hSNCA-NE | Addgene 102366 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSIN-hSNCA-NE

catalog :

102366

citations: 1

Reference
Ho P, Leung C, Liu H, Pang S, Lam C, Xian J, et al. Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA). Autophagy. 2019;:1-24 pubmed publisher

product information

Catalog Number :

102366

Product Name :

pSIN-hSNCA-NE

article :

doi	10.1080/15548627.2019.1603545
id	28205512
pubmed_id	30983487

bacterial resistance :

Ampicillin

cloning :

backbone

pSin4-EF2-IRES-Pur

backbone_mutation

backbone_origin

SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.

backbone_size

7500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

This plasmid encodes a novel 18-amino-acid protein tag - NE , which can be detected by a specific mouse monoclonal antibody in Western blotting, immunopreciptation, and immunocytochemistry. (Source of antibody: http://www.versitech.hku.hk/reagents/ne/)

growth strain :

Lentiviral overexpression of synuclein alpha

origin :

pi :

alt_names

SNCA

PD1; NACP; PARK1; PARK4

cloning

clone_method	Restriction Enzyme
cloning_site_3	SpeI
cloning_site_5	EcoRI
promoter	EF-1a
sequencing_primer_3	TTAGCTTTCGTTATCATCATAGC
sequencing_primer_5	EF-1a Forward
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	NACP, PARK1, PARK4, PD1
gene	SNCA
id	6622

genbank_ids

mutation

A silent mutation (333bp downstream of ATG) was introduced to facilitate cloning using EcoRI, but do not affect translation of wildtype protein

name

synuclein alpha

shRNA_sequence

size

477

species

9606

Homo sapiens