This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-hSNCA-NE
catalog :
102361
citations: 1
Reference
Ho P, Leung C, Liu H, Pang S, Lam C, Xian J, et al. Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA). Autophagy. 2019;:1-24 pubmed publisher
product information
Catalog Number :
102361
Product Name :
pcDNA3.1-hSNCA-NE
article :
doi10.1080/15548627.2019.1603545
id28205512
pubmed_id30983487
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid encodes a novel 18-amino-acid protein tag - NE , which can be detected by a specific mouse monoclonal antibody in Western blotting, immunopreciptation, and immunocytochemistry. (Source of antibody: http://www.versitech.hku.hk/reagents/ne/)
growth strain :
Overexpression of synuclein alpha in mammalian cells
origin :
37
pi :
alt_names
SNCA
PD1; NACP; PARK1; PARK4
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH-R
sequencing_primer_5T7 primer/CMV-F primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNACP, PARK1, PARK4, PD1
geneSNCA
id6622
genbank_ids
mutation
name synuclein alpha
shRNA_sequence
size477
species
9606
Homo sapiens
tags
locationC terminal on insert
tagNE
resistance markers :
3622
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA