This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPA016 (delta 86-130 Parkin)
catalog :
102357
citations: 1
Reference
Sauvé V, Lilov A, Seirafi M, Vranas M, Rasool S, Kozlov G, et al. A Ubl/ubiquitin switch in the activation of Parkin. EMBO J. 2015;34:2492-505 pubmed publisher
product information
Catalog Number :
102357
Product Name :
pPA016 (delta 86-130 Parkin)
article :
doi10.15252/embj.201592237
id28191881
pubmed_id26254305
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-6P1
backbone_mutation
backbone_originGE Healthcare
backbone_size4984
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Parkin is codon-optimized for expression in E. coli.
growth strain :
expresses Rat Parkin with residues 86-130 deleted
origin :
37
pi :
alt_names
PARK2
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHi
promotertac
sequencing_primer_3pGEX Rev
sequencing_primer_5pGEX Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPark, Park2
genePrkn
id56816
genbank_ids
mutationdeleted residues 86 to 130
nameParkin
shRNA_sequence
size1275
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagGST
resistance markers :
1597
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA