This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDDGFP-Leu2d-ScVrg4
catalog :
102334
citations: 2
Reference
Parker J, Corey R, Stansfeld P, Newstead S. Structural basis for substrate specificity and regulation of nucleotide sugar transporters in the lipid bilayer. Nat Commun. 2019;10:4657 pubmed publisher
Parker J, Newstead S. Structural basis of nucleotide sugar transport across the Golgi membrane. Nature. 2017;551:521-524 pubmed publisher
product information
Catalog Number :
102334
Product Name :
pDDGFP-Leu2d-ScVrg4
article :
doi10.1038/nature24464
id28192371
pubmed_id29143814
bacterial resistance :
Ampicillin
cloning :
backbonepDDGFP-Leu2d
backbone_mutation
backbone_originSimon Newstead (Addgene plasmid # 58352)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterGAL1
sequencing_primer_3CCAGTGAATAATTCTTCACC
sequencing_primer_5ATATACCTCTATACTTTAACG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYGL225W, GOG5, LDB3, VAN2, VIG4
geneVRG4
id852647
genbank_ids
mutation
nameYGL225W
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on backbone
tagGFP
resistance markers :
2005
tags :
High Copy
terms :
LEU2
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA