This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTO_CHMP2B_LAP
catalog :
101848
citations: 2
Reference
Burr M, Sparbier C, Chan K, Chan Y, Kersbergen A, Lam E, et al. An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer. Cancer Cell. 2019;36:385-401.e8 pubmed publisher
Mierzwa B, Chiaruttini N, Redondo Morata L, von Filseck J, König J, Larios J, et al. Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis. Nat Cell Biol. 2017;19:787-798 pubmed publisher
product information
Catalog Number :
101848
Product Name :
pTO_CHMP2B_LAP
article :
doi10.1038/ncb3559
id28191751
pubmed_id28604678
bacterial resistance :
Ampicillin
cloning :
backbonepTO_LAP_N-DEST
backbone_mutationCloning procedure: 1. Starting vector: pcDNA5/FRT/TO (Invitrogen) 2. Cloning of LAP tag with Gateway cloning sites: starting point mCherry_N_dest 2.1. inserted a point mutation in XbaI site to remove Dam methylation site so it can be used for cloning (from GATC to GAAG) -> pmCherry_N_dest 2.2.exchanged mCherry with GFP using KpnI and XbaI --> pLAP_N_dest 3. Inserted LAP tag and Gateway cloning site from pLAP_N_dest into pcDNA5/FRT/TO -> pTO_LAP_N-DEST
backbone_origin
backbone_size6098
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Other
Flp-FRT recombination, Tet-inducible expression
growth notes :
Part of the plasmid was derived from pmCherry_N_dest from the Helenius Lab (ETH Zuerich)
growth strain :
Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
origin :
37
pi :
alt_names
Charged Multivesicular Body Protein 2B
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5TACGGTGGGAGGTCTAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS17, CHMP2.5, DMT1, FTDALS7, VPS2-2, VPS2B
geneCHMP2B
id25978
genbank_ids
NM_014043.3
mutationsilent mutation in Thr 104; A to T
nameCHMP2B
shRNA_sequence
size641
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagLocalization and Affinity Purification (LAP) tag
resistance markers :
162
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA