AICSDP-25:MAP1LC3B-mEGFP | Addgene 101783 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

AICSDP-25:MAP1LC3B-mEGFP

catalog :

101783

citations: 2

Reference
Burr M, Sparbier C, Chan K, Chan Y, Kersbergen A, Lam E, et al. An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer. Cancer Cell. 2019;36:385-401.e8 pubmed publisher
Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua M, et al. Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell. 2017;28:2854-2874 pubmed publisher

product information

Catalog Number :

101783

Product Name :

AICSDP-25:MAP1LC3B-mEGFP

article :

doi
id	28192802
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pUC57

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

Other

Donor Template

growth notes :

This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human MAP1LC3B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .

growth strain :

Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B

origin :

pi :

alt_names

LC3B

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
gene	MAP1LC3B
id	81631

genbank_ids

mutation

homology arms contain point mutations to disrupt crRNA binding sites used, and point mutations reflecting homozygous SNPs in the WTC genome

name

MAP1LC3B Homology Arms with mEGFP-linker

shRNA_sequence

size

2729

species

9606

Homo sapiens