This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CS2 Flag-Smad2 (3A)
catalog :
101763
citations: 2
Reference
Burr M, Sparbier C, Chan K, Chan Y, Kersbergen A, Lam E, et al. An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer. Cancer Cell. 2019;36:385-401.e8 pubmed publisher
Yeh H, Hsu E, Lee S, Lang Y, Lin Y, Chang C, et al. PSPC1 mediates TGF-β1 autocrine signalling and Smad2/3 target switching to promote EMT, stemness and metastasis. Nat Cell Biol. 2018;20:479-491 pubmed publisher
product information
Catalog Number :
101763
Product Name :
CS2 Flag-Smad2 (3A)
article :
doi10.1038/s41556-018-0062-y
id28191774
pubmed_id29593326
bacterial resistance :
Ampicillin
cloning :
backbonepCS2
backbone_mutation
backbone_origin
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses SMAD2 (3A) in mammalian cells
origin :
37
pi :
alt_names
SMAD family member 2
MADH
hMAD-2
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho1
cloning_site_5EcoR1
promoterCMV
sequencing_primer_3EBV rev
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2
geneSMAD2
id4087
genbank_ids
NM_005901.5
mutationThree Ser to Ala mutations in the phosphorylation sites (S464A, S465A, S467A)
nameSMAD2
shRNA_sequence
size1400
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
plasmid copy :
This clone was modified from (Plasmid #14042) by three Ser to Ala mutations in the phosphorylation sites.
resistance markers :
3563
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA