This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTT610
catalog :
101742
citations: 3
Reference
Burr M, Sparbier C, Chan K, Chan Y, Kersbergen A, Lam E, et al. An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer. Cancer Cell. 2019;36:385-401.e8 pubmed publisher
Haruyama T, Sugano Y, Kodera N, Uchihashi T, Ando T, Tanaka Y, et al. Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy. Structure. 2019;27:152-160.e3 pubmed publisher
Sugano Y, Furukawa A, Nureki O, Tanaka Y, Tsukazaki T. SecY-SecA fusion protein retains the ability to mediate protein transport. PLoS ONE. 2017;12:e0183434 pubmed publisher
product information
Catalog Number :
101742
Product Name :
pTT610
article :
doiPONE-D-17-20168 [pii]
id28191769
pubmed_id28820900
bacterial resistance :
Ampicillin
cloning :
backbonepTV118N
backbone_mutation
backbone_originTakara
backbone_size3095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Note: The SecA version used here is Cys-less, and the amino acid sequence contains the C648S variant.
growth strain :
encoding SecY(L2V, R252G) (GGSG)4 SecA1-939 His10 fusion protein and SecE and SecG from Thermus thermophilus HB8
growth temp :
Addition of 0.4% Glucose in the media.
origin :
37
pi :
alt_names
SecYAEG
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NcoI
promoterLac
sequencing_primer_3TCTTCGCTATTACGCCAG
sequencing_primer_5AGCTCACTCATTAGGCAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesTTHA0249
genesecE
id3168489
aliasesTTHA1672
genesecY
id3169829
genbank_ids
3169354
3169944
mutationSecY(L2V, R252G) (GGSG)4 SecA1-939 His10 fusion protein, SecE and SecG
nameThermus thermophilus HB8 SecY-A/SecE/SecG
shRNA_sequence
size4666
species
32644
Other
Thermus thermophilus HB8
tags
locationC terminal on backbone
tagHis10
resistance markers :
3605
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA