pBabe-kappa-HA-dL5-myc-ADRB2 | Addgene 101253 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBabe-kappa-HA-dL5-myc-ADRB2

catalog :

101253

citations: 1

Reference
Perkins L, Yan Q, Schmidt B, Kolodieznyi D, Saurabh S, Larsen M, et al. Genetically Targeted Ratiometric and Activated pH Indicator Complexes (TRApHIC) for Receptor Trafficking. Biochemistry. 2018;57:861-871 pubmed publisher

product information

Catalog Number :

101253

Product Name :

pBabe-kappa-HA-dL5-myc-ADRB2

article :

doi
id	28191725
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE-SacLac

backbone_mutation

Sfi site removed from pBABE

backbone_origin

backbone_size

6437

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Originally used in publication: John Holleran, Dara Brown,* Margaret H. Fuhrman, Sally A. Adler, Gregory W. Fisher, and Jonathan W. Jarvik Fluorogen-Activating Proteins as Biosensors of Cell-Surface Proteins in Living Cells Cytometry A. 2010 Aug; 77(8): 776 782.

growth strain :

Expresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BsmI
cloning_site_5	EcoRI
promoter	MMLV LTR
sequencing_primer_3	BABESeqR
sequencing_primer_5	BABESeqF
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR
gene	ADRB2
id	154

genbank_ids

mutation

name

kappa-HA-dL5-myc-ADRB2

shRNA_sequence

size

1444

species

9606

Homo sapiens

4932

Saccharomyces cerevisiae