This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
catalog :
101229
citations: 1
Reference
Chen J, Dagdas Y, Kleinstiver B, Welch M, Sousa A, Harrington L, et al. Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017;550:407-410 pubmed publisher
product information
Catalog Number :
101229
Product Name :
pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
article :
doi10.1038/nature24268
id28191929
pubmed_id28931002
bacterial resistance :
Ampicillin
cloning :
backbonepCT10
backbone_mutation
backbone_originDoudna lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
growth strain :
Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
origin :
37
pi :
alt_names
Streptococcus pyogenes Cas9
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationC80S, C574S, S355C, S867C, N692A, M694A, Q695A and H698A
nameSpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
shRNA_sequence
size
species
32644
Other
Streptococcus pyogenes
tags
locationN terminal on backbone
tag10x His
locationN terminal on backbone
tagMBP
locationN terminal on backbone
tagTEV site
resistance markers :
1322
236
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA