This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSNAPf-ADR 2 Control Plasmid
catalog :
101123
citations: 2
Reference
Worboys J, Vowell K, Hare R, Ambrose A, Bertuzzi M, Conner M, et al. TIGIT can inhibit T cell activation via ligation-induced nanoclusters, independent of CD226 co-stimulation. Nat Commun. 2023;14:5016 pubmed publisher
Gutzeit V, Thibado J, Stor D, Zhou Z, Blanchard S, Andersen O, et al. Conformational dynamics between transmembrane domains and allosteric modulation of a metabotropic glutamate receptor. elife. 2019;8: pubmed publisher
product information
Catalog Number :
101123
Product Name :
pSNAPf-ADR 2 Control Plasmid
article :
doi
id28191690
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepSNAP
backbone_mutation
backbone_origin
backbone_size5925
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Originally cloned at New England Biolabs.The ADRBeta2 insert is a C-terminal insert. The signal peptide fused to the N-terminus of SNAPf is based on the 5HT3A serotonin receptor and is 72 nt. This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
growth strain :
Expression of SNAP-Tagged 2 Adrenergic Receptor in Mammalian cells
origin :
37
pi :
alt_names
ADR 2
ADRB2
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SbfI
promoterCMV
sequencing_primer_3AGGGAGTACTCACCCCAACA (ST1 Reverse Primer)
sequencing_primer_5GACGCAAATGGGCGGTAG (CMV Forward Primer)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR
geneADRB2
id154
genbank_ids
mutation
nameBeta-2-Adrenergic Receptor
shRNA_sequence
size1239
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagSNAP-tag (SNAPf)
resistance markers :
3778
3590
tags :
High Copy
terms :
Neomycin (select with G418)
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA