LLP252 pEF1a-NLS-scFvGCN4-DNMT3a | Addgene 100941 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

catalog :

100941

citations: 3

Reference
Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen T, et al. A modular dCas9-based recruitment platform for combinatorial epigenome editing. Nucleic Acids Res. 2024;52:474-491 pubmed publisher
Kojima S, Shiochi N, Sato K, Yamaura M, Ito T, Yamamura N, et al. Epigenome editing reveals core DNA methylation for imprinting control in the Dlk1-Dio3 imprinted domain. Nucleic Acids Res. 2022;50:5080-5094 pubmed publisher
Pflueger C, Tan D, Swain T, Nguyen T, Pflueger J, Nefzger C, et al. A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Genome Res. 2018;28:1193-1206 pubmed publisher

product information

Catalog Number :

100941

Product Name :

LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

article :

doi	10.1101/gr.233049.117
id	28191639
pubmed_id	29907613

bacterial resistance :

Ampicillin

cloning :

backbone

LLP245 pEF1a-TALE-DNMT3a-Wt

backbone_mutation

backbone_origin

backbone_size

4321

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

scFvGCN4 domain obtained from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene Plasmid #60904)

growth strain :

Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

origin :

pi :

alt_names

cloning

entrez_gene

aliases	DNMT3A2, HESJAS, M.HsaIIIA, TBRS
gene	DNMT3A
id	1788

genbank_ids

mutation

D10A, H840A for dCas9

name

scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain

shRNA_sequence

size

5157

species

9606

Homo sapiens

100

Synthetic