This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
LLP249 pGK-dCas9-DNMT3a V3
catalog :
100938
citations: 1
Reference
Pflueger C, Tan D, Swain T, Nguyen T, Pflueger J, Nefzger C, et al. A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Genome Res. 2018;28:1193-1206 pubmed publisher
product information
Catalog Number :
100938
Product Name :
LLP249 pGK-dCas9-DNMT3a V3
article :
doi10.1101/gr.233049.117
id28191639
pubmed_id29907613
bacterial resistance :
Ampicillin
cloning :
backboneLLP185 pLVP-dCas9-DNMT3a V2
backbone_mutation
backbone_origin
backbone_size5654
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
This V3 construct has Puro selection expressed via pEF1a promoter
growth strain :
dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesDNMT3A2, HESJAS, M.HsaIIIA, TBRS
geneDNMT3A
id1788
genbank_ids
mutationD10A, H840A for dCas9
namedCas9, DNMT3a catalytic domain
shRNA_sequence
size5157
species
9606
Homo sapiens
32644
Other
S.pyogenes
tags
locationN terminal on insert
tag3xHA
locationC terminal on insert
tag3xTy1
resistance markers :
3725
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA