LLP185 pLVP-dCas9-DNMT3a V2 | Addgene 100936 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LLP185 pLVP-dCas9-DNMT3a V2

catalog :

100936

citations: 2

Reference
Zapanta Rinonos S, Li T, Pianka S, Prins T, Eldred B, Kevan B, et al. dCas9/CRISPR-based methylation of O-6-methylguanine-DNA methyltransferase enhances chemosensitivity to temozolomide in malignant glioma. J Neurooncol. 2024;: pubmed publisher
Pflueger C, Tan D, Swain T, Nguyen T, Pflueger J, Nefzger C, et al. A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Genome Res. 2018;28:1193-1206 pubmed publisher

product information

Catalog Number :

100936

Product Name :

LLP185 pLVP-dCas9-DNMT3a V2

article :

doi	10.1101/gr.233049.117
id	28191639
pubmed_id	29907613

bacterial resistance :

Ampicillin

cloning :

backbone

pLVP

backbone_mutation

Addgene Plasmid #41816 hCas9_D10A with PCR mutagenesis to introduce the H840A mutation to generate the catalytically dead Cas9 (dCas9) and ZF-598-DNMT3A plasmid (Gift from Pilar Blancafort) was used to PCR amplify DNMT3a catalytic domain

backbone_origin

backbone_size

6235

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

CRISPR

growth strain :

dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

origin :

pi :

alt_names

cloning

entrez_gene

aliases	DNMT3A2, HESJAS, M.HsaIIIA, TBRS
gene	DNMT3A
id	1788

genbank_ids

mutation

D10A, H840A for dCas9

name

dCas9, DNMT3a catalytic domain

shRNA_sequence

size

5157

species

9606

Homo sapiens

32644

Other

S.pyogenes