This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcrDNA3.1-Chimera
catalog :
100891
citations: 1
Reference
Honeyman J, Simon E, Robine N, Chiaroni Clarke R, Darcy D, Lim I, et al. Detection of a recurrent DNAJB1-PRKACA chimeric transcript in fibrolamellar hepatocellular carcinoma. Science. 2014;343:1010-4 pubmed publisher
product information
Catalog Number :
100891
Product Name :
pcrDNA3.1-Chimera
article :
doi10.1126/science.1249484
id28191629
pubmed_id24578576
bacterial resistance :
Ampicillin
cloning :
backbonepCR3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The insert was artificially synthesized based on the major chimeric sequence found in patients
growth strain :
Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma
origin :
37
pi :
alt_names
Exon 1 of DNAJB1- Exon 2-Exon 10 of PRKACA
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3tagaaggcacagtcgagg
sequencing_primer_5cgcaaatgggcggtaggcgtg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSPF1, Hdj1, Hsp40, RSPH16B, Sis1
geneDNAJB1
id3337
aliasesCAFD1, PKACA, PPNAD4
genePRKACA
id5566
genbank_ids
mutation
nameMajor Fibrolamellar hepatocellular carcinoma Chimera
shRNA_sequence
size1221
species
9606
Homo sapiens
tags
resistance markers :
3573
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA