This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FKBP-GTSE1(638-720)-GFP
catalog :
100735
citations: 1
Reference
Wood L, Larocque G, Clarke N, Sarkar S, Royle S. New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision. J Cell Biol. 2017;216:4351-4365 pubmed publisher
product information
Catalog Number :
100735
Product Name :
FKBP-GTSE1(638-720)-GFP
article :
doi10.1083/jcb.201702188
id28191999
pubmed_id28954824
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The numbering for this plasmid is based on the longer isoform. The shorter isoform, present in this plasmid, contains amino acids 505 through 586.
growth strain :
To express a clathrin hook in mammalian cells to hot-wire endocytosis
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5AgeI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB99
geneGTSE1
id51512
genbank_ids
NM_016426.6
mutationC-terminus only
nameGTSE1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFKBP
locationC terminal on insert
tagGFP
resistance markers :
2079
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA