This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FKBP-beta2(616-951)-GFP Y215A
catalog :
100727
citations: 1
Reference
Wood L, Larocque G, Clarke N, Sarkar S, Royle S. New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision. J Cell Biol. 2017;216:4351-4365 pubmed publisher
product information
Catalog Number :
100727
Product Name :
FKBP-beta2(616-951)-GFP Y215A
article :
doi10.1083/jcb.201702188
id28191999
pubmed_id28954824
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The mutation numbering in this plasmid refers to the shorter isoform. The mutation in the longer isoform of AP2B1, which is contained in this plasmid, is Y829A.
growth strain :
To express a clathrin hook mutant in mammalian cells to hot-wire endocytosis
origin :
37
pi :
alt_names
beta2 adaptin
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5AgeI
promoterCMV
sequencing_primer_3-
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADTB2, AP105B, AP2-BETA, CLAPB1
geneAP2B1
id163
genbank_ids
BC006201
NM_001030006.1
mutationLong isoform Y215A mutation (Y815A in full length)
nameAP2B1
shRNA_sequence
size1008
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFKBP
locationC terminal on insert
tagGFP
resistance markers :
2079
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA