This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHis-hTG2
catalog :
100719
citations: 1
Reference
Han B, Cho J, Cho Y, Jeong K, Kim S, Lee B. Crystal structure of human transglutaminase 2 in complex with adenosine triphosphate. Int J Biol Macromol. 2010;47:190-5 pubmed publisher
product information
Catalog Number :
100719
Product Name :
pHis-hTG2
article :
doi10.1016/j.ijbiomac.2010.04.023
id28190088
pubmed_id20450932
bacterial resistance :
Kanamycin
cloning :
backbonepET28
backbone_mutation
backbone_originNovagen
backbone_size5900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
hTG2 has a V224G mutation that does not affect plasmid function or TG2 activity.
growth strain :
Expression of human transglutaminase 2 in E.coli
origin :
37
pi :
alt_names
transglutaminase 2
Protein-glutamine gamma-glutamyltransferase 2
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3CCGCTGAGCAATAACTAGC (T7 terminator primer)
sequencing_primer_5TAATACGACTCACTATAGGG (T7 promoter primer)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesG(h), TG(C), TGC, hTG2, tTG
geneTGM2
id7052
genbank_ids
mutationsilent mutation Arg580 (AGG--CGT) to enhance expression in E.coli
nameTGM2
shRNA_sequence
size2064
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagHis
resistance markers :
3566
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA