This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
B52 + CHEK2 sgSTOP
catalog :
100712
citations: 1
Reference
Billon P, Bryant E, Joseph S, Nambiar T, Hayward S, Rothstein R, et al. CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons. Mol Cell. 2017;67:1068-1079.e4 pubmed publisher
product information
Catalog Number :
100712
Product Name :
B52 + CHEK2 sgSTOP
article :
doi10.1016/j.molcel.2017.08.008
id28191897
pubmed_id28890334
bacterial resistance :
Ampicillin
cloning :
backboneB52 plasmid expressing CHEK2 sgSTOP and containing an empty sgRNA-expression cassette
backbone_mutation
backbone_origin
backbone_size
promoterU6 promoters
sequencing_primer_3
sequencing_primer_5GAGGTACCTCGAGGAATTCTCTAGA
vector_types
Mammalian Expression
CRISPR
growth strain :
B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoter
sequencing_primer_3
sequencing_primer_5GAGGTACCTCGAGGAATTCTCTAGA
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, TPDS4, hCds1
geneCHEK2
id11200
genbank_ids
mutation
namesgSTOP targeting CHEK2 (cloned using BbsI)
shRNA_sequencesgSTOP CHEK2: GGCCCATAATCGAGCCCAGG
size
species
9606
Homo sapiens
tags
resistance markers :
3588
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA