This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLH_Scr15
catalog :
100537
citations: 1
Reference
Hochrein L, Mitchell L, Schulz K, Messerschmidt K, Mueller Roeber B. L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast. Nat Commun. 2018;9:1931 pubmed publisher
product information
Catalog Number :
100537
Product Name :
pLH_Scr15
article :
doi10.1038/s41467-017-02208-6
id28190064
pubmed_id29789561
bacterial resistance :
Ampicillin
cloning :
backbonepL1A0*B0*_Leu
backbone_mutation
backbone_origin
backbone_size4885
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
Cre/Lox
Synthetic Biology
growth strain :
CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
origin :
37
pi :
alt_names
PhyB
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterTDH3
sequencing_primer_3CCCTGAAATTATTCCCCTAC
sequencing_primer_5GCAATAGCGCATCAAGAAAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
genePHYB
id816394
genbank_ids
mutationN-terminal version of PhyB (AA 1-621)/ Mutation of nucleotide 576 C-->A
namePhytochrome B
shRNA_sequence
size1863
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagCreN
alt_names
PIF3
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterFBA1
sequencing_primer_3AAGAAAAGAGCCGACCAA
sequencing_primer_5TTCCTTCTTCTTCGCCCA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G09530, F14J9.19, F14J9_19, PAP3, PHOTOCURRENT 1, PHYTOCHROME INTERACTING FACTOR 3, PHYTOCHROME-ASSOCIATED PROTEIN 3, POC1, phytochrome interacting factor 3, purple acid phosphatase 3
genePIF3
id837479
genbank_ids
mutation
namePhytochrome interacting factor 3
shRNA_sequence
size1572
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagSV40 NLS
locationC terminal on insert
tagCreC
resistance markers :
3478
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA