pCW57.1-MAT2A | Addgene 100521 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCW57.1-MAT2A

catalog :

100521

citations: 3

Reference
Goldberg G, Kogenaru M, Keegan S, Haase M, Kagermazova L, Arias M, et al. Engineered transcription-associated Cas9 targeting in eukaryotic cells. bioRxiv. 2023;: pubmed publisher
Russell P, Slivka J, Boyle E, Burghes A, Kearse M. Translation reinitiation after uORFs does not fully protect mRNAs from nonsense-mediated decay. RNA. 2023;29:735-744 pubmed publisher
Balderas E, Eberhardt D, Lee S, Pleinis J, Sommakia S, Balynas A, et al. Mitochondrial calcium uniporter stabilization preserves energetic homeostasis during Complex I impairment. Nat Commun. 2022;13:2769 pubmed publisher

product information

Catalog Number :

100521

Product Name :

pCW57.1-MAT2A

article :

doi	10.1126/science.aao3265
id	28190063
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pCW57.1

backbone_mutation

The original pCW57.1 vector was modified: rtTA (reverse tetracycline-controlled transactivator) was replaced with tTA (tetracycline-controlled transactivator (tTA), making this vector Tet-Off. Puromycin was replaced with Blasticidin selection

backbone_origin

Carson Thoreen at Yale University

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth strain :

Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

origin :

pi :

alt_names

AMS2

MATA2

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	pTight TRE
sequencing_primer_3
sequencing_primer_5	LNCX 5' AGCTCGTTTAGTGAACCGTCAGATC 3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MATA2, MATII, SAMS2
gene	MAT2A
id	4144

genbank_ids

mutation

name

MAT2A

shRNA_sequence

size

species

9606

Homo sapiens