pCAGGS-hPhyB-mCherry-HRasCT | Addgene 100281 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCAGGS-hPhyB-mCherry-HRasCT

catalog :

100281

citations: 1

Reference
Uda Y, Goto Y, Oda S, Kohchi T, Matsuda M, Aoki K. Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling. Proc Natl Acad Sci U S A. 2017;114:11962-11967 pubmed publisher

product information

Catalog Number :

100281

Product Name :

pCAGGS-hPhyB-mCherry-HRasCT

article :

doi	10.1073/pnas.1707190114
id	28192209
pubmed_id	29078307

bacterial resistance :

Ampicillin

cloning :

backbone

pCAGGS

backbone_mutation

backbone_origin

Junichi Miyazaki (Osaka University, Japan)

backbone_size

4870

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Expression vector of PhyB-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio. mCherry has a G229L mutation that does not affect function.

growth strain :

Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

origin :

pi :

alt_names

PhyB

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	EcoRI
promoter	CAG promoter
sequencing_primer_3	agatgctcaaggggcttcatgatg
sequencing_primer_5	catgttcatgccttcttctttttcc
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
gene	PHYB
id	816394

genbank_ids

NM_001335612.1

mutation

Human codon optimized PhyB (1-908 aa)

name

Phytochrome B

shRNA_sequence

size

species

3702

Arabidopsis thaliana