This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGL4-Cluster Promoter Delta 2
catalog :
100127
citations: 1
Reference
Segura M, Jubierre L, Li S, Soriano A, Koetz L, Gaziel Sovran A, et al. Krüppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster under physiologic and pathologic conditions. Oncotarget. 2017;8:26298-26311 pubmed publisher
product information
Catalog Number :
100127
Product Name :
pGL4-Cluster Promoter Delta 2
article :
doi10.18632/oncotarget.15459
id28190045
pubmed_id28412746
bacterial resistance :
Ampicillin
cloning :
backbonepGL4
backbone_mutation
backbone_originPromega
backbone_size4334
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
growth notes :
Addgene's sequencing results found the following discrepancies compared to the depositor's sequence: 367A>G, 739G>A, 928_929insA, 945delA, 1009C>T, 1682G>A, 2324A>T, 2503G>C, 3233A>G, 4092delG, 4784G>T. These differences could be SNPs and do not occur near the binding sites of the transcription factors of interest; thus they should not affect plasmid function.
growth strain :
It contains ~4.8 Kb of the miR-183-96-182 cluster promoter
origin :
37
pi :
alt_names
miR-182 cluster promoter
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5KpnI
promoter
sequencing_primer_3
sequencing_primer_5TAGCAAAATAGGCTGTCCCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN182, miRNA182, mir-182
geneMIR182
id406958
genbank_ids
mutation
namemiR-183-96-82 Cluster promoter 4.8Kb Fragment
shRNA_sequence
size4732
species
9606
Homo sapiens
tags
resistance markers :
3554
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA