This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28-his-3C_Clu3B6p
catalog :
100093
citations: 1
Reference
Sluchanko N, Tugaeva K, Greive S, Antson A. Chimeric 14-3-3 proteins for unraveling interactions with intrinsically disordered partners. Sci Rep. 2017;7:12014 pubmed publisher
product information
Catalog Number :
100093
Product Name :
pET28-his-3C_Clu3B6p
article :
doi10.1038/s41598-017-12214-9 [pii]
id28191940
pubmed_id28931924
bacterial resistance :
Kanamycin
cloning :
backbonepET28-his-3C
backbone_mutation
backbone_origin
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The 3C site was introduced immediately prior to 14-3-3 sequence to facilitate purification by subtractive IMAC.
growth strain :
expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide
origin :
37
pi :
alt_names
14-3-3 sigma fusion
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYWHAS
geneSFN
id2810
genbank_ids
mutation75-77 residues changed to AAA to facilitate crystallization
name14-3-3 sigma human
shRNA_sequence
size780
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHSPB6 peptide
locationN terminal on insert
tagHis6
resistance markers :
3551
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA