This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28-his-3C_Tau352
catalog :
100092
citations: 1
Reference
Tugaeva K, Tsvetkov P, Sluchanko N. Bacterial co-expression of human Tau protein with protein kinase A and 14-3-3 for studies of 14-3-3/phospho-Tau interaction. PLoS ONE. 2017;12:e0178933 pubmed publisher
product information
Catalog Number :
100092
Product Name :
pET28-his-3C_Tau352
article :
doiPONE-D-16-49989 [pii]
id28190035
pubmed_id28575131
bacterial resistance :
Kanamycin
cloning :
backbonepET28-his-3C
backbone_mutation
backbone_origin
backbone_size5231
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The 3C site is introduced in front of Tau sequence for easy purification by subtractive IMAC
growth strain :
Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag
origin :
37
pi :
alt_names
Tau
MAP-Tau
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
geneMAPT
id4137
genbank_ids
mutationwild type sequence
nameFetal microtubule-associated protein tau
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHis6
resistance markers :
3551
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA