This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DNMT3A-dCas9
catalog :
100090
citations: 4
Reference
Woods M, Weiss A, Sokol A, Graumann J, Boettger T, Richter A, et al. Epigenetically silenced apoptosis-associated tyrosine kinase (AATK) facilitates a decreased expression of Cyclin D1 and WEE1, phosphorylates TP53 and reduces cell proliferation in a kinase-dependent manner. Cancer Gene Ther. 2022;29:1975-1987 pubmed publisher
O Geen H, Tomková M, Combs J, Tilley E, Segal D. Determinants of heritable gene silencing for KRAB-dCas9 + DNMT3 and Ezh2-dCas9 + DNMT3 hit-and-run epigenome editing. Nucleic Acids Res. 2022;50:3239-3253 pubmed publisher
Deutschmeyer V, Breuer J, Walesch S, Sokol A, Graumann J, Bartkuhn M, et al. Epigenetic therapy of novel tumour suppressor ZAR1 and its cancer biomarker function. Clin Epigenetics. 2019;11:182 pubmed publisher
O Geen H, Ren C, Nicolet C, Perez A, Halmai J, Le V, et al. dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression. Nucleic Acids Res. 2017;45:9901-9916 pubmed publisher
product information
Catalog Number :
100090
Product Name :
DNMT3A-dCas9
article :
doi10.1093/nar/gkx578
id28192203
pubmed_id28973434
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.3-TOPO
backbone_mutation
backbone_originThermoFisher
backbone_size5407
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3polyA TK-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDNMT3A2, HESJAS, M.HsaIIIA, TBRS
geneDNMT3A
id1788
genbank_ids
NP_072046.2
mutationincludes aa 602-912
nameDNMT3A
shRNA_sequence
size
species
9606
Homo sapiens
tags
location
tag3XFLag-NLS-DNMT3A-dCas9-NLS
resistance markers :
1166
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA