RNA Pol II CTD phospho Tyr1 antibody (mAb) | Active Motif 61383 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

RNA Pol II CTD phospho Tyr1 antibody (mAb)

catalog :

61383

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3D12

reactivity :

human, brewer's yeast

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; brewer's yeast; fig S1	Harlen K, Trotta K, Smith E, Mosaheb M, Fuchs S, Churchman L. Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue. Cell Rep. 2016;15:2147-2158 pubmed publisher
western blot; human; 1:200; fig 2	Zaborowska J, Baumli S, Laitem C, O Reilly D, Thomas P, O Hare P, et al. Herpes Simplex Virus 1 (HSV-1) ICP22 protein directly interacts with cyclin-dependent kinase (CDK)9 to inhibit RNA polymerase II transcription elongation. PLoS ONE. 2014;9:e107654 pubmed publisher

product information

Catalog Number :

61383

Product Name :

RNA Pol II CTD phospho Tyr1 antibody (mAb)

Host Species :

Rat

Clonality :

Monoclonal

Antigen Modification :

Phosphorylated

Size :

100 ug

Clone :

3D12

Isotype :

IgG1

CrossReactivity :

Human, Other (Wide Range)

Background :

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at sites within the heptapeptide repeat, at serine 2, serine 5, serine 7 and tyrosine 1. RNA pol II Tyrosine 1 phosphorylation is found during elongation and appears to activate transcription by suppressing RNA pol II termination.

Immunogen :

This antibody was raised against a synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.

Uses :

ChIP, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: The use of this antibody is described in the following Reference:ChIP, IP & WB: Mayer, A. et al. (2012) Science 336:1723-1725.