Histone H3K4me3 antibody (mAb) | Active Motif 61379 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K4me3 antibody (mAb)

catalog :

61379

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MABI 0304

reactivity :

human, thale cress

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; thale cress immunohistochemistry; thale cress	Xu Y, Gan E, Zhou J, Wee W, Zhang X, Ito T. Arabidopsis MRG domain proteins bridge two histone modifications to elevate expression of flowering genes. Nucleic Acids Res. 2014;42:10960-74 pubmed publisher
	Gomes A, Ilter D, Low V, Endress J, Fernández García J, Rosenzweig A, et al. Age-induced accumulation of methylmalonic acid promotes tumour progression. Nature. 2020;585:283-287 pubmed publisher
	Han M, Wei G, McManus C, Hillier L, Reinke V. Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression. BMC Genomics. 2019;20:500 pubmed publisher
	Yue M, Ogawa A, Yamada N, Charles Richard J, Barski A, Ogawa Y. Xist RNA repeat E is essential for ASH2L recruitment to the inactive X and regulates histone modifications and escape gene expression. PLoS Genet. 2017;13:e1006890 pubmed publisher

product information

Catalog Number :

61379

Product Name :

Histone H3K4me3 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

100 ug

Clone :

MABI 0304

Isotype :

IgG1

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Immunogen :

This Histone H3 trimethyl Lys4 antibody was raised against a synthetic peptide containing trimethyl Lys4 of human histone H3.

Uses :

IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody was created as described in Kimura, H. et al. (2008) Cell Struct Func 33: 61-73.