RNA Pol II CTD phospho Thr4 antibody (mAb) | Active Motif 61361 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

RNA Pol II CTD phospho Thr4 antibody (mAb)

catalog :

61361

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

6D7

reactivity :

human, brewer's yeast

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; brewer's yeast; fig S1	Harlen K, Trotta K, Smith E, Mosaheb M, Fuchs S, Churchman L. Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue. Cell Rep. 2016;15:2147-2158 pubmed publisher

product information

Catalog Number :

61361

Product Name :

RNA Pol II CTD phospho Thr4 antibody (mAb)

Host Species :

Rat

Clonality :

Monoclonal

Antigen Modification :

Phosphorylated

Size :

100 ug

Clone :

6D7

Isotype :

IgG2b

CrossReactivity :

Human, Other (Wide Range)

Background :

RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.

Immunogen :

This antibody was raised against a synthetic peptide corresponding to RNA Pol II CTD phosphorylated at threonine 4.

Uses :

ChIP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: The use of this antibody is described in the following References:ChIP & WB: Mayer, A. et al. (2012) Science 336:1723-1725. Hintermair, C. et al. (2012) EMBO J 31:2784-2797.