Histone H4ac (pan-acetyl) antibody (mAb) | Active Motif 61337 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H4ac (pan-acetyl) antibody (mAb)

catalog :

61337

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3HH4-3H10

reactivity :

human

application :

western blot, chromatin immunoprecipitation

citations: 1

Reference
Mustafi S, Camarena V, Volmar C, Huff T, Sant D, Brothers S, et al. Vitamin C Sensitizes Melanoma to BET Inhibitors. Cancer Res. 2018;78:572-583 pubmed publisher

product information

Catalog Number :

61337

Product Name :

Histone H4ac (pan-acetyl) antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Acetylated

Size :

100 ug

Clone :

3HH4-3H10

Isotype :

IgG1k

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.

Immunogen :

This antibody was raised against a peptide corresponding to amino acid residue(s) 1- 24 of human histone H4 acetylated at lysines 5, 8, 12 and 16. Due to sequence similarity, cross-reactivity has been observed with acetylated Histone H2B.

Uses :

ChIP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: The use of this antibody is described in the following reference:ChIP: Dilworth, F.J. et al. (2000) Mol Cell 6:1049-1058.