MeCP2 antibody (mAb) | Active Motif 61285 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

MeCP2 antibody (mAb)

catalog :

61285

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4B4

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 3

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; mouse; loading ...; fig 3k	Liu Y, Zhang B, Meng X, Korn M, Parent J, Lu L, et al. UHRF2 regulates local 5-methylcytosine and suppresses spontaneous seizures. Epigenetics. 2017;12:551-560 pubmed publisher
immunohistochemistry; rat; 1:200	Hoefflin S, Carter D. Neuronal expression of SOX2 is enriched in specific hypothalamic cell groups. J Chem Neuroanat. 2014;61-62:153-60 pubmed publisher
	Tai D, Liu Y, Hsu W, Ma Y, Cheng S, Liu S, et al. MeCP2 SUMOylation rescues Mecp2-mutant-induced behavioural deficits in a mouse model of Rett syndrome. Nat Commun. 2016;7:10552 pubmed publisher

product information

Catalog Number :

61285

Product Name :

MeCP2 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

100 ug

Clone :

4B4

Isotype :

IgG2a

CrossReactivity :

Human, Mouse, Rat

Background :

Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl CpG binding protein 2, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.

Immunogen :

This MeCP2 antibody was raised against a full-length protein corresponding to rat MeCP2.

Uses :

ChIP, IF, IHC, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: The use of this antibody is described in the following references: ChIP, IP, IF, IHC & WB: Jost KL, et al. (2011) PLoS One 6 (11): e26499