Histone H3, C-terminal antibody (pAb) | Active Motif 61277 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3, C-terminal antibody (pAb)

catalog :

61277

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, brewer's yeast, Xenopus laevis

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; brewer's yeast; loading ...; fig 2d	Yu R, Sun L, Sun Y, Han X, Qin L, Dang W. Cellular response to moderate chromatin architectural defects promotes longevity. Sci Adv. 2019;5:eaav1165 pubmed publisher
western blot; mouse; fig 6 western blot; Xenopus laevis; fig 1	Powers N, Parvanov E, Baker C, Walker M, Petkov P, Paigen K. The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo. PLoS Genet. 2016;12:e1006146 pubmed publisher
western blot; mouse; fig 2	Carofino B, Ayanga B, Tracey L, Brooke Bisschop T, Justice M. PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL. Biol Open. 2016;5:645-53 pubmed publisher
western blot; human	Fan H, Zhang H, Pascuzzi P, Andrisani O. Hepatitis B virus X protein induces EpCAM expression via active DNA demethylation directed by RelA in complex with EZH2 and TET2. Oncogene. 2016;35:715-26 pubmed publisher

product information

Catalog Number :

61277

Product Name :

Histone H3, C-terminal antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Size :

100 ug

Isotype :

IgG

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Immunogen :

This Histone H3, C-terminal antibody was raised against a C-terminal peptide of histone H3.

Uses :

ChIP, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.