5-Carboxylcytosine (5-caC) antibody (pAb) | Active Motif 61225 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

5-Carboxylcytosine (5-caC) antibody (pAb)

catalog :

61225

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, other

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig s5c	Shen Q, Zhang Q, Shi Y, Shi Q, Jiang Y, Gu Y, et al. Tet2 promotes pathogen infection-induced myelopoiesis through mRNA oxidation. Nature. 2018;554:123-127 pubmed publisher
other; human; 1:5000; fig 1a	McLaughlin D, Coey C, Yang W, Drohat A, Matunis M. Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo. J Biol Chem. 2016;291:9014-24 pubmed publisher
	Weber A, Krawczyk C, Robertson A, KuÅ›nierczyk A, VÃ¥gbÃ¸ C, Schuermann D, et al. Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism. Nat Commun. 2016;7:10806 pubmed publisher

product information

Catalog Number :

61225

Product Name :

5-Carboxylcytosine (5-caC) antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

100 ul

Isotype :

Serum

CrossReactivity :

Mouse, Other (Not Species Specific)

Background :

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.

Immunogen :

This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.

Uses :

DB, IF

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: The use of this antibody is described in the following references:DB: Inoue A et al. (2011) Cell Res. 21: 1670-1676.IF: Inoue A et al. (2011) Cell Res. 21: 1670-1676.