Histone H3.X/Y antibody (mAb) | Active Motif 61161 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3.X/Y antibody (mAb)

catalog :

61161

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

8H6-2111

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; loading ...; fig 4c, 4g, s3d	Taubenschmid Stowers J, Rostovskaya M, Santos F, Ljung S, Argelaguet R, Krueger F, et al. 8C-like cells capture the human zygotic genome activation program in vitro. Cell Stem Cell. 2022;29:449-459.e6 pubmed publisher
immunocytochemistry; human; loading ...; fig 3b western blot; human; loading ...; fig 3c	Resnick R, Wong C, Hamm D, Bennett S, Skene P, Hake S, et al. DUX4-Induced Histone Variants H3.X and H3.Y Mark DUX4 Target Genes for Expression. Cell Rep. 2019;29:1812-1820.e5 pubmed publisher

product information

Catalog Number :

61161

Product Name :

Histone H3.X/Y antibody (mAb)

Host Species :

Rat

Clonality :

Monoclonal

Size :

100 ug

Clone :

8H6-2111

Isotype :

IgG2a

CrossReactivity :

Human

Background :

Histones H3.X and H3.Y are primate-specific variants of histone H3. H3.X and H3.Y are roughly 80% conserved compared to histone H3.3 The H3.Y protein is highly expressed in several primary cell lines, cancer cell lines and human brain. Expression of histone H3.Y is induced by stress. Exogenously expressed H3.X and H3.Y are efficiently incorporated into chromatin and exhibit dynamics similar to canonical histone H3. Knockdown of H3.Y results in slowed growth and altered expression of cell cycle related genes.

Immunogen :

This H3.X/Y antibody was raised against a synthetic peptide corresponding to amino acids 9 - 20 of human H3.X.

Uses :

IF, IHC, WB

Storage :

Antibodies in solution can be stored at -80M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used in the following references:IF: Wiedemann SM et al. (2010) J Cell Bio 190: 777-791.WB: Wiedemann SM et al. (2010) J Cell Bio 190: 777-791.