CtIP antibody (mAb) | Active Motif 61141 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

CtIP antibody (mAb)

catalog :

61141

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

14-1

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig s6a	Phillips A, Millet A, Tigano M, Dubois S, Crimmins H, Babin L, et al. Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion. Mol Cell. 2017;65:527-538.e6 pubmed publisher
western blot; human; loading ...; fig 2b	Shamanna R, Lu H, de Freitas J, Tian J, Croteau D, Bohr V. WRN regulates pathway choice between classical and alternative non-homologous end joining. Nat Commun. 2016;7:13785 pubmed publisher
immunocytochemistry; human; loading ...; fig 3b western blot; human; loading ...; fig 4b	Bakr A, KÃ¶cher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, et al. Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Oncotarget. 2016;7:57679-57693 pubmed publisher
immunocytochemistry; human; loading ...; fig 4e	Kais Z, Rondinelli B, Holmes A, O Leary C, Kozono D, D Andrea A, et al. FANCD2 Maintains Fork Stability in BRCA1/2-Deficient Tumors and Promotes Alternative End-Joining DNA Repair. Cell Rep. 2016;15:2488-99 pubmed publisher
western blot; human; fig 1	Onyango D, Howard S, Neherin K, Yanez D, Stark J. Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination. Nucleic Acids Res. 2016;44:5702-16 pubmed publisher
immunocytochemistry; human; fig 7	Nagy Z, Kalousi A, Furst A, Koch M, Fischer B, Soutoglou E. Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs. PLoS Genet. 2016;12:e1005791 pubmed publisher
immunocytochemistry; human; 1:500; fig 4	Zhang H, Liu H, Chen Y, Yang X, Wang P, Liu T, et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016;7:10201 pubmed publisher
western blot; human	Zhou Y, Caron P, Legube G, Paull T. Quantitation of DNA double-strand break resection intermediates in human cells. Nucleic Acids Res. 2014;42:e19 pubmed publisher
	Chen Y, Liu H, Zhang H, Sun C, Hu Z, Tian Q, et al. And-1 coordinates with CtIP for efficient homologous recombination and DNA damage checkpoint maintenance. Nucleic Acids Res. 2017;45:2516-2530 pubmed publisher

product information

Catalog Number :

61141

Product Name :

CtIP antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

100 ug

Clone :

14-1

Isotype :

IgG1

CrossReactivity :

Human

Background :

CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.

Immunogen :

This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.

Uses :

IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used in the following references: IF: Zhang Y and Jasin M (20011) Nat Struct Mol Biol 16: 80-85. IP: Xu Y et al. (2006) Genes Dev 20: 1721-1726. WB: Xu Y et al. (2006) Genes Dev 20: 1721-1726.