Histone H3K36me3 antibody (pAb) | Active Motif 61101 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K36me3 antibody (pAb)

catalog :

61101

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, Xenopus laevis

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig e4c	Weinberg D, Papillon Cavanagh S, Chen H, Yue Y, Chen X, Rajagopalan K, et al. The histone mark H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape. Nature. 2019;573:281-286 pubmed publisher
ChIP-Seq; human; loading ...; fig 5b	Stewart E, McEvoy J, Wang H, Chen X, Honnell V, Ocarz M, et al. Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses. Cancer Cell. 2018;34:411-426.e19 pubmed publisher
western blot; Xenopus laevis; fig 1 ChIP-Seq; mouse; tbl 1 western blot; mouse; fig 6	Powers N, Parvanov E, Baker C, Walker M, Petkov P, Paigen K. The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo. PLoS Genet. 2016;12:e1006146 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 2a immunocytochemistry; human; 1:1000; fig s3d western blot; human; loading ...; fig 1a	Fang D, Gan H, Lee J, Han J, Wang Z, Riester S, et al. The histone H3.3K36M mutation reprograms the epigenome of chondroblastomas. Science. 2016;352:1344-8 pubmed publisher
chromatin immunoprecipitation; mouse; fig s11b western blot; mouse; fig s8a	Lu C, Jain S, Hoelper D, Bechet D, Molden R, Ran L, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352:844-9 pubmed publisher
chromatin immunoprecipitation; mouse; fig 6	Lee B, Wu C, Lin Y, Park S, Wei L. Synergistic activation of Arg1 gene by retinoic acid and IL-4 involves chromatin remodeling for transcription initiation and elongation coupling. Nucleic Acids Res. 2016;44:7568-79 pubmed publisher
	Haggerty C, Kretzmer H, Riemenschneider C, Kumar A, Mattei A, Bailly N, et al. Dnmt1 has de novo activity targeted to transposable elements. Nat Struct Mol Biol. 2021;28:594-603 pubmed publisher
	Zhang Y, Fang D. The incorporation loci of H3.3K36M determine its preferential prevalence in chondroblastomas. Cell Death Dis. 2021;12:311 pubmed publisher
	Zaidan N, Sridharan R. HP1γ regulates H3K36 methylation and pluripotency in embryonic stem cells. Nucleic Acids Res. 2020;48:12660-12674 pubmed publisher
	Fitz James M, Tong P, Pidoux A, Özadam H, Yang L, White S, et al. Large domains of heterochromatin direct the formation of short mitotic chromosome loops. elife. 2020;9: pubmed publisher
	Stojic L, Lun A, Mascalchi P, Ernst C, Redmond A, Mangei J, et al. A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division. Nat Commun. 2020;11:1851 pubmed publisher
	Erkek S, Johann P, Finetti M, Drosos Y, Chou H, Zapatka M, et al. Comprehensive Analysis of Chromatin States in Atypical Teratoid/Rhabdoid Tumor Identifies Diverging Roles for SWI/SNF and Polycomb in Gene Regulation. Cancer Cell. 2018;: pubmed publisher
	Fang D, Wang H, Zhang Z. Probing the Function of Oncohistones Using Mutant Transgenes and Knock-In Mutations. Methods Mol Biol. 2018;1832:339-356 pubmed publisher
	Chen X, Zhang M, Gan H, Wang H, Lee J, Fang D, et al. A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma. Nat Commun. 2018;9:2949 pubmed publisher
	Chiang Y, Park I, Terzo E, Tripathi D, Mason F, Fahey C, et al. SETD2 Haploinsufficiency for Microtubule Methylation Is an Early Driver of Genomic Instability in Renal Cell Carcinoma. Cancer Res. 2018;78:3135-3146 pubmed publisher
	Janevska S, Baumann L, Sieber C, Munsterkotter M, Ulrich J, Kämper J, et al. Elucidation of the Two H3K36me3 Histone Methyltransferases Set2 and Ash1 in Fusarium fujikuroi Unravels Their Different Chromosomal Targets and a Major Impact of Ash1 on Genome Stability. Genetics. 2018;208:153-171 pubmed publisher
	Pünzeler S, Link S, Wagner G, Keilhauer E, Kronbeck N, Spitzer R, et al. Multivalent binding of PWWP2A to H2A.Z regulates mitosis and neural crest differentiation. EMBO J. 2017;36:2263-2279 pubmed publisher
	Aldiri I, Xu B, Wang L, Chen X, Hiler D, Griffiths L, et al. The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis. Neuron. 2017;94:550-568.e10 pubmed publisher
	Ho T, Park I, Zhao H, Tong P, Champion M, Yan H, et al. High-resolution profiling of histone h3 lysine 36 trimethylation in metastatic renal cell carcinoma. Oncogene. 2016;35:1565-74 pubmed publisher

product information

Catalog Number :

61101

Product Name :

Histone H3K36me3 antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

100 ul

Isotype :

IgG

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.

Immunogen :

This Histone H3 trimethyl Lys36 antibody was raised against a peptide containing trimethyl Lys36 of human histone H3.

Uses :

ChIP, ChIP-chip, ChIP-Seq, DB, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.