RNA pol II CTD phospho Ser5 antibody (mAb) | Active Motif 61085 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

RNA pol II CTD phospho Ser5 antibody (mAb)

catalog :

61085

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

300000000

reactivity :

human, brewer's yeast, fruit fly

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; fig 1s7a	Mittleman B, Pott S, Warland S, Zeng T, Mu Z, Kaur M, et al. Alternative polyadenylation mediates genetic regulation of gene expression. elife. 2020;9: pubmed publisher
western blot; fruit fly ; loading ...; fig s3b	Nil Z, Herv s R, Gerbich T, Leal P, Yu Z, Saraf A, et al. Amyloid-like Assembly Activates a Phosphatase in the Developing Drosophila Embryo. Cell. 2019;178:1403-1420.e21 pubmed publisher
western blot; human; loading ...; fig 1d	Muhar M, Ebert A, Neumann T, Umkehrer C, Jude J, Wieshofer C, et al. SLAM-seq defines direct gene-regulatory functions of the BRD4-MYC axis. Science. 2018;360:800-805 pubmed publisher
western blot; human; loading ...; fig 6a	Winter G, Mayer A, Buckley D, Erb M, Roderick J, Vittori S, et al. BET Bromodomain Proteins Function as Master Transcription Elongation Factors Independent of CDK9 Recruitment. Mol Cell. 2017;67:5-18.e19 pubmed publisher
immunoprecipitation; brewer's yeast; fig S1	Harlen K, Trotta K, Smith E, Mosaheb M, Fuchs S, Churchman L. Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue. Cell Rep. 2016;15:2147-2158 pubmed publisher
ChIP-Seq; human; loading ...; fig 4	Ramos M, Wijetunga N, McLellan A, Suzuki M, Greally J. DNA demethylation by 5-aza-2'-deoxycytidine is imprinted, targeted to euchromatin, and has limited transcriptional consequences. Epigenetics Chromatin. 2015;8:11 pubmed publisher
chromatin immunoprecipitation; human	Singh A, Compe E, Le May N, Egly J. TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription. Am J Hum Genet. 2015;96:194-207 pubmed publisher
chromatin immunoprecipitation; human	Eifler T, Shao W, Bartholomeeusen K, Fujinaga K, JÃ¤ger S, Johnson J, et al. Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts. Mol Cell Biol. 2015;35:468-78 pubmed publisher

product information

Catalog Number :

61085

Product Name :

RNA pol II CTD phospho Ser5 antibody (mAb)

Host Species :

Rat

Clonality :

Monoclonal

Antigen Modification :

Phosphorylated

Size :

100 ug

Clone :

300000000

Isotype :

IgG2a

CrossReactivity :

Human

Background :

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

Immunogen :

This RNA pol II CTD phospho Ser5 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.

Uses :

ChIP, ChIP-chip, ChIP-Seq, IF, IP, WB

Storage :

Antibodies in solution can be stored at -80M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used in the following references:ChIP: Chapman RD et al. (2007) Science 318: 1780-1782.IF: Chapman RD et al. (2007) Science 318: 1780-1782.IP: Chapman RD et al. (2007) Science 318: 1780-1782.WB: Chapman RD et al. (2007) Science 318: 1780-1782.