RNA pol II CTD phospho Ser2 antibody (mAb) | Active Motif 61083 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

RNA pol II CTD phospho Ser2 antibody (mAb)

catalog :

61083

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3.0e+10

reactivity :

fission yeast, human, mouse, brewer's yeast

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, ChIP-Seq

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human immunohistochemistry; human	Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
western blot; human; loading ...; fig 1d	Muhar M, Ebert A, Neumann T, Umkehrer C, Jude J, Wieshofer C, et al. SLAM-seq defines direct gene-regulatory functions of the BRD4-MYC axis. Science. 2018;360:800-805 pubmed publisher
western blot; human; loading ...; fig 6a	Winter G, Mayer A, Buckley D, Erb M, Roderick J, Vittori S, et al. BET Bromodomain Proteins Function as Master Transcription Elongation Factors Independent of CDK9 Recruitment. Mol Cell. 2017;67:5-18.e19 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 5h western blot; mouse; 1:1000; loading ...; fig 3e	Strikoudis A, Lazaris C, Trimarchi T, Galvao Neto A, Yang Y, Ntziachristos P, et al. Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a. Nat Cell Biol. 2016;18:1127-1138 pubmed publisher
immunoprecipitation; brewer's yeast; fig S1	Harlen K, Trotta K, Smith E, Mosaheb M, Fuchs S, Churchman L. Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue. Cell Rep. 2016;15:2147-2158 pubmed publisher
western blot; mouse; fig 4	Chung H, Xu C, Fuchs A, Mund A, Lange M, Staege H, et al. PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3. elife. 2016;5: pubmed publisher
chromatin immunoprecipitation; mouse; fig 4b	Garcia Cuellar M, Büttner C, Bartenhagen C, Dugas M, Slany R. Leukemogenic MLL-ENL Fusions Induce Alternative Chromatin States to Drive a Functionally Dichotomous Group of Target Genes. Cell Rep. 2016;15:310-22 pubmed publisher
chromatin immunoprecipitation; fission yeast; fig 8	Catania S, Pidoux A, Allshire R. Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin. PLoS Genet. 2015;11:e1004986 pubmed publisher

product information

Catalog Number :

61083

Product Name :

RNA pol II CTD phospho Ser2 antibody (mAb)

Host Species :

Rat

Clonality :

Monoclonal

Antigen Modification :

Phosphorylated

Size :

100 ug

Clone :

3.0e+10

Isotype :

IgG1

CrossReactivity :

Human

Background :

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.

Immunogen :

This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.

Uses :

ChIP, ChIP-chip, ChIP-Seq, IF, IP, WB

Storage :

Antibodies in solution can be stored at -80M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used in the following references:ChIP: Chapman RD et al. (2007) Science 318: 1780-1782.IF: Chapman RD et al. (2007) Science 318: 1780-1782.IP: Chapman RD et al. (2007) Science 318: 1780-1782.WB: Chapman RD et al. (2007) Science 318: 1780-1782.