RNA pol II antibody (mAb) | Active Motif 61081 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

RNA pol II antibody (mAb)

catalog :

61081

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

4F8

reactivity :

fission yeast, human

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 3

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; fission yeast; fig 7	Catania S, Pidoux A, Allshire R. Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin. PLoS Genet. 2015;11:e1004986 pubmed publisher
chromatin immunoprecipitation; human; fig s6 western blot; human; fig s6	Xing Z, Lin A, Li C, Liang K, Wang S, Liu Y, et al. lncRNA directs cooperative epigenetic regulation downstream of chemokine signals. Cell. 2014;159:1110-1125 pubmed publisher
	Reeder J, Kwak Y, McNamara R, Forst C, D Orso I. HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells. elife. 2015;4: pubmed publisher

product information

Catalog Number :

61081

Product Name :

RNA pol II antibody (mAb)

Host Species :

Rat

Clonality :

Monoclonal

Size :

100 ug

Clone :

4F8

Isotype :

IgG1

CrossReactivity :

Human

Background :

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Immunogen :

This RNA pol II antibody was raised against a synthetic peptide corresponding to human RNA pol II C-terminal heptad repeat domain.

Uses :

ChIP, IF, IP, WB

Storage :

Antibodies in solution can be stored at -80M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used in the following references: ChIP: Chapman RD et al. (2007) Science 318: 1780-1782. IF: Chapman RD et al. (2007) Science 318: 1780-1782. IP: Chapman RD et al. (2007) Science 318: 1780-1782. WB: Chapman RD et al. (2007) Science 318: 1780-1782.