Histone H3K56ac antibody (mAb) | Active Motif 61061 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K56ac antibody (mAb)

catalog :

61061

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12.1

reactivity :

human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2a	Zhang X, Li B, Rezaeian A, Xu X, Chou P, Jin G, et al. H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis. Nat Commun. 2017;8:14799 pubmed publisher
western blot; human; loading ...; fig 2a	Pal S, Graves H, Ohsawa R, Huang T, Wang P, Harmacek L, et al. The Commercial Antibodies Widely Used to Measure H3 K56 Acetylation Are Non-Specific in Human and Drosophila Cells. PLoS ONE. 2016;11:e0155409 pubmed publisher
western blot; human	Drogaris P, Villeneuve V, Pomiès C, Lee E, Bourdeau V, Bonneil E, et al. Histone deacetylase inhibitors globally enhance h3/h4 tail acetylation without affecting h3 lysine 56 acetylation. Sci Rep. 2012;2:220 pubmed publisher
	Tessarz P, Santos Rosa H, Robson S, Sylvestersen K, Nelson C, Nielsen M, et al. Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification. Nature. 2014;505:564-8 pubmed publisher

product information

Catalog Number :

61061

Product Name :

Histone H3K56ac antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Acetylated

Size :

100 ug

Clone :

12.1

Isotype :

IgG1

CrossReactivity :

Budding Yeast, Fission Yeast, Human

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Lysine N-M-NM-5-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.

Immunogen :

This Histone H3 acetyl Lys56 antibody was raised against a peptide containing acetyl-Lys56 of human histone H3.

Uses :

ChIP, DB, IP

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.