Histone H3K36me3 antibody (mAb) | Active Motif 61021 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K36me3 antibody (mAb)

catalog :

61021

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MABI 0333

reactivity :

human, thale cress

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4a	Papillon Cavanagh S, Lu C, Gayden T, Mikael L, Bechet D, Karamboulas C, et al. Impaired H3K36 methylation defines a subset of head and neck squamous cell carcinomas. Nat Genet. 2017;49:180-185 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200; loading ...; fig s3	Chen Y, Xu J, Skanderup A, Dong Y, Brannon A, Wang L, et al. Molecular analysis of aggressive renal cell carcinoma with unclassified histology reveals distinct subsets. Nat Commun. 2016;7:13131 pubmed publisher
chromatin immunoprecipitation; thale cress immunohistochemistry; thale cress	Xu Y, Gan E, Zhou J, Wee W, Zhang X, Ito T. Arabidopsis MRG domain proteins bridge two histone modifications to elevate expression of flowering genes. Nucleic Acids Res. 2014;42:10960-74 pubmed publisher
	DERY K, Silver C, Yang L, Shively J. Interferon regulatory factor 1 and a variant of heterogeneous nuclear ribonucleoprotein L coordinately silence the gene for adhesion protein CEACAM1. J Biol Chem. 2018;293:9277-9291 pubmed publisher
	Powers N, Parvanov E, Baker C, Walker M, Petkov P, Paigen K. The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo. PLoS Genet. 2016;12:e1006146 pubmed publisher
	Tiedemann R, Hlady R, Hanavan P, Lake D, Tibes R, Lee J, et al. Dynamic reprogramming of DNA methylation in SETD2-deregulated renal cell carcinoma. Oncotarget. 2016;7:1927-46 pubmed publisher

product information

Catalog Number :

61021

Product Name :

Histone H3K36me3 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

100 ug

Clone :

MABI 0333

Isotype :

IgG1

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.

Immunogen :

This Histone H3 trimethyl Lys36 antibody was raised against a peptide containing trimethyl Lys36 of human Histone H3.

Uses :

ChIP, DB, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: The creation of this antibody was described in Kimura, H. et al. (2008) Cell Struct Func 33: 61-73.This antibody has been used in the following references: ChIP: Rechtsteiner A et al. (2010) PLoS Genet 6: e1001091.IF: Rechtsteiner A et al. (2010) PLoS Genet 6: e1001091.