Histone H3K36me2 antibody (mAb) | Active Motif 61019 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K36me2 antibody (mAb)

catalog :

61019

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MABI 0332

reactivity :

human, roundworm

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; roundworm ; 1:200; loading ...; fig 6b	Amendola P, Zaghet N, Ramalho J, Vilstrup Johansen J, Boxem M, Salcini A. JMJD-5/KDM8 regulates H3K36me2 and is required for late steps of homologous recombination and genome integrity. PLoS Genet. 2017;13:e1006632 pubmed publisher
chromatin immunoprecipitation; human; fig s9 western blot; human; fig 2	García Carpizo V, Sarmentero J, Han B, Grana O, Ruiz Llorente S, Pisano D, et al. NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation. Sci Rep. 2016;6:32952 pubmed publisher

product information

Catalog Number :

61019

Product Name :

Histone H3K36me2 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

100 ug

Clone :

MABI 0332

Isotype :

IgG1

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.

Immunogen :

This Histone H3 dimethyl Lys36 antibody was raised against a peptide containing dimethyl Lys36 of human Histone H3.

Uses :

ChIP, DB, TR-FRET, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: The creation of this antibody was described in Kimura, H. et al. (2008) Cell Struct Func 33: 61-73.This antibody has been used in the following references: ChIP: Rechtsteiner A et al. (2010) PLoS Genet 6: e1001091.